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M94A0636.TXT
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1994-10-21
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Document 0636
DOCN M94A0636
TI HIV-1 integration in peripheral blood monocytes and cultured
macrophages.
DT 9412
AU Sonza S; Maerz A; Mills J; Crowe S; AIDS Pathogenesis Research Unit,
NCHVR, Macfarlane Burnet Centre; for Medical Research, Fairfield,
Victoria.
SO Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:66 (abstract no. TB9).
Unique Identifier : AIDSLINE ASHM5/94349019
AB Although integration has been clearly demonstrated in HIV-1 infected
lymphocytes, there has been only a single unconvincing report of this in
primary macrophages. We have used various polymerase chain
reaction-based techniques to determine whether and when this step in the
infection process takes place in monocytes and macrophages. A novel
procedure, Alu-PCR, which amplifies DNA between HIV-1 and ubiquitous Alu
repeat sequences, indicated that while integrated DNA could not be
detected even after 7 days following infection of freshly isolated
monocytes with HIV-1Ba-L, it was detectable within 24 h of infection of
cells which had been cultured for as little as one day. Greatest levels
were detected at the peak of infection. This finding correlates with
other work in our laboratory that monocytes are usually refractory to in
vitro infection (measured by both p24 antigen production and initiation
of reverse transcription) on the day on which they are isolated but
become increasingly more susceptible to infection with time in culture.
This phenomenon is not related to the level of CD4 on the cells but
possibly to virus entry.
DE Human HIV-1/*GENETICS/PATHOGENICITY In Vitro
Macrophages/*MICROBIOLOGY Monocytes/*MICROBIOLOGY Polymerase Chain
Reaction Virulence/GENETICS Virus Integration/*GENETICS Virus
Replication/*GENETICS MEETING ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).